Saddest Moment in Lost

Going along with the official Lostpedia rewatch, I just watched "Deus Ex Machina". The episode where Locke reconnects with his father only to have his heart broken when he learns it was just a ploy to get his kidney. I thnk the saddest moment in Lost is when Locke pulls up to his fathers house and he isn't allowed in. He drives away crying and screaming and then it cuts back to the island and he is pounding on the hatch door screaming out to the island asking "Why it was doing this?"

I was wondering what some of the moments were that you guys thought was the saddest or most emotional?

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